Fig. 10

LncRNA expression validation and 3-lncRNA centered TTR regulatory network. a The 10 TTR-lncRNAs expression fold change detected with RNA-Seq and qRT-PCR, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. b A 3-lncRNA centered TTR regulatory network. 3 TTR-lncRNAs were shown in diamond nodes, TTR-mRNAs in circle nodes and miRNAs in triangle. Equal dash edges indicate trans relationship. Yellow separate arrow pointing from CBFTA2T2 to MSTRG.15047.3 indicates upstream nearby location. Yellow parallel edges between FRMD6-AS2 and FRMD6 represent overlapping and antisense location. Sinewave edges between TTR-lncRNAs, TTR-mRNAs with miRNAs denotes potential ceRNA relationship. TTR-mRNAs filled in green (PPP2R5C, UBE2Nand ASB), purple (MCM7), brown (TAF6) and blue (FGF13 and PBRM1) indicates genes in sub-network 1–4. c The expression of lncRNA MSTRG.15047.3, AC008403.3 and FRMD6-AS2 were examined with qRT-PCR in aqueous humor and serum samples from 30 DR patients and 10 healthy participants. * P < 0.05, ** P < 0.01, *** P < 0.001