Fig. 4

Function enrichment analyses of TTR-mRNAs and DE-mRNAs. KEGG pathway and GO-biological process (BP), cellular component (CC) and molecular function (MF) annotation and enrichment analysis and Gene Set Enrichment Analysis (GSEA) were used to study the function of 853 TTR-mRNAs. a TOP 15 significant enriched KEGG pathways like apoptosis, Glucagon signaling pathway, cAMP signaling pathway, MAPK signaling pathway, ErbB signaling pathway. b TOP 15 significant enriched GO biological processes like chromosome segregation, regulation of cell cycle, positive regulation of I-κB kinase/NF-κB signaling and cellular response to DNA damage stimulus. c GSEA analysis of DE-mRNAs in high glucose group compared with low glucose group identified Cell cycle related process like DNA replication and inflammatory pathways like IL2-STAT5. d GSEA analysis of DE-mRNAs in TTR group compared with high glucose group not only identified pathways involved in cell cycle like G2/M checkpoint, cell apoptosis pathways like Fas pathway, neovascularization like ERBB1/4 receptor pathway and oxidative stress response like Nitric Oxide Stimulates Guanylate Cyclase and P38-MKK3 pathway