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Fig. 2 | BMC Medical Genomics

Fig. 2

From: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

Fig. 2

Discordant results were validated by NGS and Sanger sequencing. a-c Representative images of read alignments (BAM files) of sample #1 (a), #2 (b) and #3 (c) were visualized with Ion Reporter Genome Viewer (upper images). PCR products produced by BNA-clamp PCR were purified and used as templates for Sanger sequencing. Sequencing chromatograms show the mutations in each sample (lower image). Arrows indicate the position of the mutations. a In sample #1, KRAS p.Q61K was detected by NGS. At this site, multi-nucleotide variants (c.180_181delTCinsAA) existed in codons 60 and 61. b In sample #2, there was no apparent variant at codon 12 of NRAS. We did not observe an amplification plot signal by real-time PCR and obtained no visible PCR product for subsequent Sanger sequencing analysis. c Two different mutations (NRAS p.G12D and KRAS p.A146T) were observed in sample #3

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