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Table 1 Comparison of KRAS and NRAS mutation status determined by PCR-rSSO, BNA-clamp PCR and NGS

From: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

 PCR-rSSOBNA-clamp PCRDeep sequencing by NGS
Sample No.Nucleotide changesDeduced amino acid changesNucleotide changesDeduced amino acid changesNucleotide changesDeduced amino acid changes
#1Wild-typeWild-typec.180_181delTCinsAAKRAS Q61Kc.180_181delTCinsAAKRAS Q61K
#2c.34G > TNRAS G12CWild-typeWild-typeWild-typeWild-type
#3c.436G > AKRAS A146Tc.436G > AKRAS A146Tc.436G > AKRAS A146T
Wild-typeWild-typec.35G > ANRAS G12Dc.35G > ANRAS G12D
#4c.35G > CKRAS G12Ac.35G > CKRAS G12Ac.35G > CKRAS G12A
#5c.35G > CKRAS G12Ac.35G > CKRAS G12Ac.35G > CKRAS G12A
#6c.35G > CKRAS G12Ac.35G > CKRAS G12Ac.35G > CKRAS G12A
#7c.34G > TKRAS G12Cc.34G > TKRAS G12Cc.34G > TKRAS G12C
#8c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#9c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#10c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#11c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#12c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#13c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#14c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#15c.35G > AKRAS G12Dc.35G > AKRAS G12Dc.35G > AKRAS G12D
#16c.34G > AKRAS G12Sc.34G > AKRAS G12Sc.34G > AKRAS G12S
#17c.34G > AKRAS G12Sc.34G > AKRAS G12Sc.34G > AKRAS G12S
#18c.35G > TKRAS G12 Vc.35G > TKRAS G12 Vc.35G > TKRAS G12 V
#19c.35G > TKRAS G12 Vc.35G > TKRAS G12 Vc.35G > TKRAS G12 V
#20c.35G > TKRAS G12 Vc.35G > TKRAS G12 Vc.35G > TKRAS G12 V
#21c.38G > AKRAS G13Dc.38G > AKRAS G13Dc.38G > AKRAS G13D
#22c.38G > AKRAS G13Dc.38G > AKRAS G13Dc.38G > AKRAS G13D
#23c.38G > AKRAS G13Dc.38G > AKRAS G13Dc.38G > AKRAS G13D
#24c.38G > AKRAS G13Dc.38G > AKRAS G13Dc.38G > AKRAS G13D
#25c.38G > AKRAS G13Dc.38G > AKRAS G13Dc.38G > AKRAS G13D
#26c.182A > TKRAS Q61Lc.182A > TKRAS Q61Lc.182A > TKRAS Q61L
#27c.436G > AKRAS A146Tc.436G > AKRAS A146Tc.436G > AKRAS A146T
#28c.35G > ANRAS G12Dc.35G > ANRAS G12Dc.35G > ANRAS G12D
#29c.38G > TNRAS G13 Vc.38G > TNRAS G13 Vc.38G > TNRAS G13 V
#30c.181C > ANRAS Q61Kc.181C > ANRAS Q61Kc.181C > ANRAS Q61K
#31c.181C > ANRAS Q61Kc.181C > ANRAS Q61Kc.181C > ANRAS Q61K
#32c.182A > GNRAS Q61Rc.182A > GNRAS Q61Rc.182A > GNRAS Q61R
#33NANAc.1799 T > ABRAF V600Ec.1799 T > ABRAF V600E
#34NANAc.1799 T > ABRAF V600Ec.1799 T > ABRAF V600E
#35NANAc.1799 T > ABRAF V600Ec.1799 T > ABRAF V600E
#36–50Wild-typeWild-typeWild-typeWild-typeWild-typeWild-type
  1. NA not applicable