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Table 1 Comparison of KRAS and NRAS mutation status determined by PCR-rSSO, BNA-clamp PCR and NGS

From: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

 

PCR-rSSO

BNA-clamp PCR

Deep sequencing by NGS

Sample No.

Nucleotide changes

Deduced amino acid changes

Nucleotide changes

Deduced amino acid changes

Nucleotide changes

Deduced amino acid changes

#1

Wild-type

Wild-type

c.180_181delTCinsAA

KRAS Q61K

c.180_181delTCinsAA

KRAS Q61K

#2

c.34G > T

NRAS G12C

Wild-type

Wild-type

Wild-type

Wild-type

#3

c.436G > A

KRAS A146T

c.436G > A

KRAS A146T

c.436G > A

KRAS A146T

Wild-type

Wild-type

c.35G > A

NRAS G12D

c.35G > A

NRAS G12D

#4

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

#5

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

#6

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

c.35G > C

KRAS G12A

#7

c.34G > T

KRAS G12C

c.34G > T

KRAS G12C

c.34G > T

KRAS G12C

#8

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#9

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#10

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#11

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#12

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#13

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#14

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#15

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

c.35G > A

KRAS G12D

#16

c.34G > A

KRAS G12S

c.34G > A

KRAS G12S

c.34G > A

KRAS G12S

#17

c.34G > A

KRAS G12S

c.34G > A

KRAS G12S

c.34G > A

KRAS G12S

#18

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

#19

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

#20

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

c.35G > T

KRAS G12 V

#21

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

#22

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

#23

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

#24

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

#25

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

c.38G > A

KRAS G13D

#26

c.182A > T

KRAS Q61L

c.182A > T

KRAS Q61L

c.182A > T

KRAS Q61L

#27

c.436G > A

KRAS A146T

c.436G > A

KRAS A146T

c.436G > A

KRAS A146T

#28

c.35G > A

NRAS G12D

c.35G > A

NRAS G12D

c.35G > A

NRAS G12D

#29

c.38G > T

NRAS G13 V

c.38G > T

NRAS G13 V

c.38G > T

NRAS G13 V

#30

c.181C > A

NRAS Q61K

c.181C > A

NRAS Q61K

c.181C > A

NRAS Q61K

#31

c.181C > A

NRAS Q61K

c.181C > A

NRAS Q61K

c.181C > A

NRAS Q61K

#32

c.182A > G

NRAS Q61R

c.182A > G

NRAS Q61R

c.182A > G

NRAS Q61R

#33

NA

NA

c.1799 T > A

BRAF V600E

c.1799 T > A

BRAF V600E

#34

NA

NA

c.1799 T > A

BRAF V600E

c.1799 T > A

BRAF V600E

#35

NA

NA

c.1799 T > A

BRAF V600E

c.1799 T > A

BRAF V600E

#36–50

Wild-type

Wild-type

Wild-type

Wild-type

Wild-type

Wild-type

  1. NA not applicable
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