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Table 2 Quality and coverage depth of next generation sequencing

From: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

SampleMapped readsOn targetMean depthUniformity
#13,896,30898%17,98498%
#22,686,90398%12,26296%
#31,202,87098%505780%
#42,101,69798%946792%
#52,069,59394%893599%
#62,623,48898%11,77590%
#72,950,28997%13,05699%
#81,834,91798%822787%
#93,270,77298%14,83696%
#103,957,90699%18,101100%
#112,980,02698%13,450100%
#121,518,77098%686486%
#132,569,73991%10,930100%
#143,089,05399%14,112100%
#153,471,55799%15,934100%
#162,385,27298%10,83290%
#172,990,67099%14,066100%
#182,803,95498%12,871100%
#191,818,39397%808699%
#20649,51097%279492%
#213,236,15699%14,95099%
#222,215,85498%9819100%
#232,600,28999%11,94298%
#242,487,26797%11,07796%
#252,127,00698%955585%
#261,227,27068%334175%
#272,386,36095%10,36699%
#283,042,16298%13,66595%
#293,321,95298%14,75696%
#302,905,12398%12,93492%
#312,437,11498%10,95890%
#323,031,33997%13,56698%
#332,819,33598%12,76693%
#342,335,61098%10,53282%
#353,136,40197%14,13898%
#362,583,62597%11,88599%
#371,987,56196%888697%
#381,161,90995%518198%
#391,592,15398%733797%
#401,421,96698%649091%
#411,716,68398%785493%
#422,121,08599%992097%
#431,970,85198%892899%
#442,510,67099%11,60899%
#451,398,96298%623199%
#462,872,51797%13,06398%
#471,760,82897%792897%
#482,259,83297%991590%
#491,948,99998%892199%
#503,134,12698%14,28796%
  1. Mapped reads: number of sequencing reads that were mapped to the human genome
  2. On target: percentage of mapped reads that were aligned over the target region
  3. Mean depth: Average base coverage depth over all bases targeted in the reference
  4. Uniformity: percentage of target bases covered by at least 0.2x the average base read depth