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Table 2 Quality and coverage depth of next generation sequencing

From: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

Sample

Mapped reads

On target

Mean depth

Uniformity

#1

3,896,308

98%

17,984

98%

#2

2,686,903

98%

12,262

96%

#3

1,202,870

98%

5057

80%

#4

2,101,697

98%

9467

92%

#5

2,069,593

94%

8935

99%

#6

2,623,488

98%

11,775

90%

#7

2,950,289

97%

13,056

99%

#8

1,834,917

98%

8227

87%

#9

3,270,772

98%

14,836

96%

#10

3,957,906

99%

18,101

100%

#11

2,980,026

98%

13,450

100%

#12

1,518,770

98%

6864

86%

#13

2,569,739

91%

10,930

100%

#14

3,089,053

99%

14,112

100%

#15

3,471,557

99%

15,934

100%

#16

2,385,272

98%

10,832

90%

#17

2,990,670

99%

14,066

100%

#18

2,803,954

98%

12,871

100%

#19

1,818,393

97%

8086

99%

#20

649,510

97%

2794

92%

#21

3,236,156

99%

14,950

99%

#22

2,215,854

98%

9819

100%

#23

2,600,289

99%

11,942

98%

#24

2,487,267

97%

11,077

96%

#25

2,127,006

98%

9555

85%

#26

1,227,270

68%

3341

75%

#27

2,386,360

95%

10,366

99%

#28

3,042,162

98%

13,665

95%

#29

3,321,952

98%

14,756

96%

#30

2,905,123

98%

12,934

92%

#31

2,437,114

98%

10,958

90%

#32

3,031,339

97%

13,566

98%

#33

2,819,335

98%

12,766

93%

#34

2,335,610

98%

10,532

82%

#35

3,136,401

97%

14,138

98%

#36

2,583,625

97%

11,885

99%

#37

1,987,561

96%

8886

97%

#38

1,161,909

95%

5181

98%

#39

1,592,153

98%

7337

97%

#40

1,421,966

98%

6490

91%

#41

1,716,683

98%

7854

93%

#42

2,121,085

99%

9920

97%

#43

1,970,851

98%

8928

99%

#44

2,510,670

99%

11,608

99%

#45

1,398,962

98%

6231

99%

#46

2,872,517

97%

13,063

98%

#47

1,760,828

97%

7928

97%

#48

2,259,832

97%

9915

90%

#49

1,948,999

98%

8921

99%

#50

3,134,126

98%

14,287

96%

  1. Mapped reads: number of sequencing reads that were mapped to the human genome
  2. On target: percentage of mapped reads that were aligned over the target region
  3. Mean depth: Average base coverage depth over all bases targeted in the reference
  4. Uniformity: percentage of target bases covered by at least 0.2x the average base read depth