Fig. 1
From: RNA-seq from archival FFPE breast cancer samples: molecular pathway fidelity and novel discovery
RNA quantity and quality are impacted by epithelial area and block age. a) Five separate archival FFPE blocks (each a different color and symbol) of breast cancer tissue were subjected to RNA isolation from 10 to 40 μm of tissue and evaluated for overall RNA yield and b) RNA quality determined by DV200 value. c) 58 archival breast cancer tissues from two different repositories (indicated by green and blue dots) were subjected to RNA isolation from one 10 μm section and evaluated for the relationship between DV200 and year of collection, (black line is best fit line using a centered fourth order polynomial) and d) overall RNA yield. e) Epithelial area and f) specimen type compared to yield. Red lines at 100 ng and DV200 = 30 denote recommended minimal technical limits for successful sequencing