Fig. 1

The effect of MEG3 in OA chondrocyte proliferation, apoptosis and cartilage matrix degradation. a, the expression of MEG3 in normal group and OA group detected by qRT-PCR; data expressed as mean ± standard deviation (SD). b, expression of MEG3 by qRT-PCR after transfection of OA chondrocytes with pcDNA3.1-MEG3 or si-MEG3. c, CCK-8 showed the proliferation of cells transfected with si-MEG3; MEG3 knockdown inhibited the proliferation of OA chondrocytes. d, the proliferation of cells after pcDNA3.1-MEG3 transfection detected by CCK-8 assay. e, the expression of PCNA and marker Ki67 was detected by western blot after transfection with pcDNA3.1-MEG3 or si-MEG3 in OA chondrocytes. f, cell apoptosis of OA chondrocytes was detected by flow cytometry. g, the expression of apoptosis-related proteins Bcl-2 and Bax in OA chondrocytes was measured by western blot; h, Western blot was performed to assess the expression of cartilage matrix proteins (MMP13, ADAMTS-5, Collagen II, Aggrecan) in chondrocytes of each group. *, P < 0.05 compared with Blank or pcDNA3.1-NC group. & P < 0.05, compared with the Blank or si-NC group