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Table 1 Primer sequences of the qMSP assays

From: The values of AHCY and CBS promoter methylation on the diagnosis of cerebral infarction in Chinese Han population

Gene

Primer sequence(5′- 3′)

Fragment size

Annealing temperature

AHCY-F

GGTCGTAATCGGTTGTAG

124 bp

58 °C

AHCY-R

CAATTCCTATTCCCAAACTAAA

  

CBS-F

GGATGGAGTTATATTATGAAGGT

93 bp

56 °C

CBS-R

AACAATCTCGCTCAATCG

  

ACTB-F(1)

TGGTGATGGAGGAGGTTTAGTAAGT

133 bp

58 °C

ACTB-R(1)

AACCAATAAAACCTACTCCTCCCTTAA

  

ACTB-F(2)

GTGATGGAGGAGGTTTAGTAAGTT

129 bp

56 °C

ACTB-R(2)

CCAATAAAACCTACTCCTCCCTTAA

  
  1. F indicates the qMSP upstream primer; R indicates the qMSP downstream primer; (1) indicates the ACTB primer sequence at 58 °C, and (2) indicates the ACTB primer sequence at 56 °C
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BMC Medical Genomics

ISSN: 1755-8794

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