Fig. 4

Effect of increasingly stringent alternative allele frequency and coverage thresholds on sensitivity and precision of variant calling. Each curve represents a different algorithm. Each dot on each curve represents a different threshold, ranging from requiring alternative allele frequency AF ≥0.01 and coverage ≥20 reads (least stringent) to AF ≥0.1 and coverage ≥200 reads (most stringent). a Averaged results across all TST170 datasets. b Averaged results across all OF datasets