Fig. 5

Results of cDNA Array analysis of ACC gene expression. a Heat-map and unsupervised hierarchical clustering based on the 769 genes that differ most between ACC and NA (\(\sigma /\sigma \mathrm{max}\) >0.2, p < 0.05, q = 0.18, > 2-FC, one sample per patient, probe-sets collapsed to median per gene). Compared to the normal adrenal, 289 DEG were noted to be upregulated, and 480 downregulated in the tumors. The ACC samples cluster separately from the normal adrenal (NA) samples, with the exception of 35 T, 47 T, and 48 T (gray arrow) that cluster together with the NA samples (Note: as these tumor samples were from metastasectomies normal adrenal tissue could not have contaminated the samples). b Heat-map and unsupervised hierarchical clustering based on the 33 out of 576 Tier 1 COSMIC cancer census genes that differ the most between ACC and NA
(\(\sigma /\sigma \mathrm{max}\) >0.2, p < 0.05, q = 0.17, > 2-FC). The ACC samples cluster separately from the NA. Again, 35 T, 47 T, and 48 T cluster together with the NA samples