Fig. 3

Changes in the expression of tissue-specific signatures with gestational age. For each tissue, the expression of the top 20 most-specific genes (based on the Gene Atlas dataset) was transformed into a Z-score and averaged in each AF sample. A Robust Locally Weighted Regression and Smoothing Scatterplots (LOESS) model fit through the Z-scores as a function of gestational age is shown using lines (see Fig. S2 for individual values). Tissue signature trends are set to have the same value at 16 weeks of gestation. Differentially expressed tissue signatures were sorted by the magnitude of change from 16 to 41 weeks of gestation and the top 10 tissues with increased (a) and deceased (b) expression are shown. AF, amniotic fluid