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Table 2 Effect of covariates on amniotic fluid transcriptome. Early midtrimester is defined as gestational age 16.4–21.0 weeks and late midtrimester is defined as gestational age 21.1–24.0 weeks. Differential splicing was assessed only for contrasts with significant differential expression

From: Amniotic fluid cell-free transcriptome: a glimpse into fetal development and placental cellular dynamics during normal pregnancy

Effect

Comparison/sample size

Adjustment variable

Diff. expressed Genes

Diff. spliced Genes

In-silico validation

Gestational age effect during midtrimester

Late midtrimester (n = 15) vs. Early Midtrimester (n = 15)

Fetal sex

413

806

 

Gestational age (overall)

TNL (n = 68) vs. Midtrimester (n = 30)

Fetal sex

6194

8566

Hui et al. 2013 [98].; Kamath-Rayne et al. 2015 [85]

Mode of collection in TNL

Cesarean delivery (n = 64) versus transabdominal amniocentesis (n = 4)

Fetal sex

0

0

 

Smoking

Smoker (n = 17) vs. non-smoker (n = 79)

Gestational age and fetal sex

0

0

 

Fetal sex

Male (n = 51) vs. Female (n = 47)

Gestational age

252

240

Larrabee et al. 2005 [83]

Maternal race

Caucasian (n = 6) vs. African American (n = 82)

Gestational age and fetal sex

0

0

 

Parity

Parous (n = 87) vs. Nulliparous (n = 11)

Gestational age and fetal sex

0

0

 

Obesity (overall)

Obese (n = 38) vs. Lean (n = 33)

Gestational age and fetal sex

0

0

Edlow et al. 2014 [99]

Obesity in midtrimester

Obese(n = 8) vs. Lean (n = 11)

Gestational age and fetal sex

0

0

Edlow et al. 2014 [99]

Indication for amniocentesis during midtrimester*

Detection of infection/inflammation (n = 17) vs. assessing fetal karyotype (n = 9)

Gestational age and fetal sex

0

0

 
  1. TNL Term not in labor. Indications for both