Fig. 2

STAiR18 transcript architecture in INA-6 cells. a Schematic representation of STAiR18 transcript variants identified by CAPTURE-RNA-sequencing. Already annotated exons are shown in dark blue, novel exons in light blue. Exons targeted by the siRNAs and regions covered by the biotinylated oligonucleotides are marked at the top and bottom of the scheme, respectively. b Validation of abundant STAiR18 splice variants in INA-6 by qPCR using transcript-specific primers pairs. Values were normalized to U6 RNA (n = 4). c Knockdown (KD) of STAiR18 isoforms in INA-6 cells using siRNAs targeting STAiR18 exons (Figure 2a) and a negative control siRNA. KD efficiency was determined 48 h after KD by qPCR using intron spanning primer pairs. Values were normalized to U6 RNA (n = 3)