Fig. 4

Identification of genes regulated by STAiR18 and STAT3. a Differentially regulated STAT3 and STAiR18 target genes determined after STAiR18-Ex1 and STAT3 KD (n = 4). RNA was isolated 40 h post-transfection and subjected to gene expression microarrays. A minimal fold-change of 1.5-fold and maximum p-value of 0.05 were applied as cutoff criteria, yielding 545 and 721 differentially regulated candidates upon STAT3 and STAiR18 knockdown, respectively. 58 of these candidates are regulated by both knockdowns. b The fold-changes of 58 genes differentially regulated by both the KD of STAT3 and STAiR18 were plotted against each other. c Validation of selected transcripts regulated by both KDs by qPCR using specific primer pairs. Values were normalized to U6 RNA (n = 4). The detected expression of equivalent genes identified by microarray is shown at the top panel (i) for comparison