Skip to main content
Fig. 1 | BMC Medical Genomics

Fig. 1

From: Absolute measurement of the tissue origins of cell-free DNA in the healthy state and following paracetamol overdose

Fig. 1

Validation of ddPCR assays on gDNA containing 1lox or 2lox alleles. a 1lox ddPCR assay specifically amplified gDNA containing 1lox alleles, b 2lox ddPCR assay amplified gDNA containing 2lox alleles with less than 0.1% of positive droplets from 1lox gDNA. The pink line shows the threshold between positive and negative droplets at fluorescence amplitude 3000. c Dilution experiment showed both assays robustly detected less than 30 targets. The estimated number of targets refers to the number of successful amplifications as indicated by positive droplets in ddPCR following Poisson correction to estimate the real input of DNA fragment. Each dot showed mean of 1lox or 2lox copies measured by ddPCR assays in six replicates. Error bars showed standard error

Back to article page

BMC Medical Genomics

ISSN: 1755-8794

Contact us