Fig. 2

Cytogenetic features at diagnosis and relapse. a Image of the FISH results with the BCR/ABL1 probe revealing an additional green signal from the BCR locus on chromosome 22. Images of metaphases FISH with the TCF3/PBX/HLF probe in samples from diagnosis (b) and relapse (c). Image of interphase FISH with CCP13/CCP21 probe in samples from diagnosis (d) revealing a lack of 13q deletion genes: MED4, ITM2B, RB1, RCBTB2, and CYSLTR2). FISH was performed on cells in metaphase using probes (Cytocell Ltd., Oxford Gene Technology, Cambridge, United Kingdom) according to the manufacturer’s recommendations. Images were captured by an Olympus BX41TF microscope equipped with a Jenoptik camera and analysed with Isis Software (MetaSystems)