Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case–control study

Table 4 Primer sequences used for genotyping in ARMS-PCR

SNPs	Primers	Sequences	Primer Length (bp)	PCR products (bp)
rs6265	FO	CTACAGTTCCACCAGGTGAGAAGAGTG	27	400
	RO	ATGGACATGTTTGCAGCATCTAGGTA	26	400
	FI (c)	TGGTCCTCATCCAACAGCTCTTCTATaAC	29	253
	RI(t)	TTGGCTGACACTTTCGAACcCA	22	201
rs713729	FO	CTTTCTAGCGGAATCCCGACTGTG	24	407
	RO	CAGTGAAAAAGCAAACCAGGACACTG	26	407
	FI(a)	CTTCTCATTCTTGAGGTCTGACCCACA	27	196
	RI(t)	AGGTGGTCAGAAAGCCCCTCAGA	23	265
rs2076369	FO	CATGTTGCCCAAGCTGGTCTCAAACTC	27	299
	RO	CTGGACACCCGTAACTGCTCTGACC	35	299
	FI(g)	AGGAGTCTCAGTCCAGAACAGTCTTGACG	29	191
	RI(t)	CTCCACACCCTGAGCCCCTTCTCA	24	165

Expected product size in bps depending on the SNPs. FO: Forward outer primer; FI: Forward inner primer; RI: Reverse inner primer; RO: Reverse outer primer; bp: Base pairs; ARMS-PCR: Amplification refractory mutation system-polymerase chain reaction; SNP: Single nucleotide polymorphism

ISSN: 1755-8794