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Table 5 The ARMS-PCRs condition for targeted SNPs

From: Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case–control study

SNPs Primers First Denaturation 35 cycles Last extension
Tm°C Min Denaturation Annealing Extension Tm°C Min
   Tm°C Min Tm°C Min Tm°C Min
rs6265 FO 94 °C 7 min 94 °C 30 s 61.5 25 s 72 °C 45 s 72 °C 7 min
RO
FI (c)
RI(t)
rs713729 FO 61 30 s 30 s 5 min
RO
FI(a)
RI(t)
rs2076369 FO 64 25 s 30 s 5 min
RI(t)
FI(g) 67 30 s
RO
  1. FO: Forward outer primer; FI: Forward inner primer; RI: Reverse inner primer; RO: Reverse outer primer; TM: Temperature; Min: Minute; S: Seconds; °C: Centigrade; ARMS-PCR: Amplification refractory mutation system-polymerase chain reaction; SNP: Single nucleotide polymorphism