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Fig. 5 | BMC Medical Genomics

Fig. 5

From: Integrated transcriptome and proteome analyses identify novel regulatory network of nucleus pulposus cells in intervertebral disc degeneration

Fig. 5

Functional study of candidate genes in degenerated NP primary cells. a The efficiency of TNFAIP6, CHI3L1, KRT19, DPT, COL6A2, and COL11A2 knockdown were examined in normal NP cells. NC group represents NP cell transfection using a scramble siRNA that showed no potential target to these genes. The RNA expression level of NP ECM related anabolic genes (ACAN, COL2 and CHSY1) and catabolic genes (MMP3, MMP13, ADAMTS4) were examined in TNFAIP6 (b), CHI3L1 (c), KRT19 (d), DPT (e), COL6A2 (f), and COL11A2 (g) knockdown NP cells using real-time PCR method. RNA level of GAPDH was used as internal reference. All Data are represented as mean ± SD, *p < 0.05, *p < 0.01

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