Table 1 Comparison of technical parameters
From: Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation
Assay | Input material | Input amount (ng) | No. of samples/run | Sequencer | Flow cell | Costs library preparation/sample | Duration of library preparation (days) | Duration of sequencing run (h) |
|---|---|---|---|---|---|---|---|---|
Archer FusionPlex Lung Panel (Archer DX) | tNA | 200 | 16 | MiSeq (Illumina) | MiSeq Reagent Kit v2 (300 cycles) | 200 € | 2 | 26 |
QIAseq Targeted RNAscan Custom Panel (Qiagen) | RNA | 200 | 23 | NextSeq 500 (Illumina) | NextSeq 500/550 High Output Kit v2.1 (300 cycles) | 190 € | 2 | 29 |
Oncomine Focus Assay (Thermo Fisher Scientific) | RNA | 10–50 | 20–40 | Ion S5 System or Ion PGM System (Thermo Fisher Scientic) | Ion 520 or Ion 316 Chip | 130–150 € | 1.5 | 3 |
TruSight Tumor 170 Assay (Illumina) | RNA | 85 | 20 | NextSeq 500 (Illumina) | NextSeq 500/550 Output Kit v2.1 (300 cycles) | 160 € | 2 | 24 |
SureSelect XT HS Custom Panel (Agilent) | DNA | 200 | 23 | NextSeq 500 (Illumina) | NextSeq 500/550 High Output Kit v2.1 (300 cycles) | 320 € | 2 | 24 |