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Table 1 Comparison of technical parameters

From: Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation

Assay Input material Input amount (ng) No. of samples/run Sequencer Flow cell Costs library preparation/sample Duration of library preparation (days) Duration of sequencing run (h)
Archer FusionPlex Lung Panel (Archer DX) tNA 200 16 MiSeq (Illumina) MiSeq Reagent Kit v2 (300 cycles) 200 € 2 26
QIAseq Targeted RNAscan Custom Panel (Qiagen) RNA 200 23 NextSeq 500 (Illumina) NextSeq 500/550 High Output Kit v2.1 (300 cycles) 190 € 2 29
Oncomine Focus Assay (Thermo Fisher Scientific) RNA 10–50 20–40 Ion S5 System or Ion PGM System (Thermo Fisher Scientic) Ion 520 or Ion 316 Chip 130–150 € 1.5 3
TruSight Tumor 170 Assay (Illumina) RNA 85 20 NextSeq 500 (Illumina) NextSeq 500/550 Output Kit v2.1 (300 cycles) 160 € 2 24
SureSelect XT HS Custom Panel (Agilent) DNA 200 23 NextSeq 500 (Illumina) NextSeq 500/550 High Output Kit v2.1 (300 cycles) 320 € 2 24
  1. tNA total nucleic acid