Fig. 3

Sample clustering and network construction of the weighted co-expressed genes. a Clustering dendrogram of 9 calcified and 8 normal aortic valve samples. The color intensity was proportional to disease status (calcified or normal aortic valve) or age. Analysis of b the scale-free fit index and c the mean connectivity for various soft-thresholding powers. The soft-thresholding power of 12 was selected based on the scale‑free topology criterion. d Dendrogram clustered based on a dissimilarity measure (1-TOM). Gene expression similarity is assessed by a pair-wise weighted correlation metric and clustered based on a topological overlap metric into modules. Each color below represents one co-expression module, and every branch stands for one gene. e The cluster dendrogram of module eigengenes