Fig. 2

Differential expression analysis of RNA-seq data. A, B Volcano plot of statistically significant DEGs at FDR < 0.05 for the B7 versus WT group (A) and the C12 versus WT group (B). The red dots represent DEGs at FDR < 0.01 [i.e., − log10(padj) ≥ 2], with an absolute value of log2FoldChange ≥ 1. C, D Venn diagrams showing the DEGs identified from the B7 versus WT (B7) and C12 versus WT (C12) groups at FDR < 0.05 (C) and FDR < 0.05 with an absolute value of log2FoldChange ≥ 1(D). E Top 20 DEGs in the B7 group. The expression levels of the same genes in the C12 group is also included. The horizontal arrow indicates that FDR value decreases from left to right