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Fig. 4 | BMC Medical Genomics

Fig. 4

From: RNA-seq analysis reveals significant transcriptome changes in huntingtin-null human neuroblastoma cells

Fig. 4

Cell proliferation was reduced in B7 HttKO cells. A, B Representative light microscopic images showing the cell morphology of WT (A), B7 (B) and C12 (C) cells. scale bar: 100 μm. C Cell proliferation rate was determined by daily cell counting for up to 4 days using the trypan blue exclusion method. D Cell viability measurement for up to 4 days using the trypan blue exclusion method. E Real-time monitoring cell proliferation by impedance measurement. Cells were seeded at 1 × 104 cells per well. Cell adherence, spreading and proliferation were monitored every 15 min for 165 h using the xCELLigence real time cell analysis system. N = 4 for each cell type. F Real-time monitoring cell proliferation by impedance measurement. Cells were seeded at 3 × 104 cells per well. Cell adherence, spreading and proliferation were monitored every 15 min for 30 h using the xCELLigence real time cell analysis system. N = 2 for each cell type

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