Table 1 The primer sequence of STR loci
From: DMD/BMD prenatal diagnosis and treatment expectation in a single centre in China for 15 years
Probe name | Length (in bp) | Forward/reverse primer (5′–3′) |
|---|---|---|
5′-(CA)n-3′ | 206–228 | tcttgatatatagggattatttgtgtttgttatac/attatgaaactataaggaataactcatttagc |
DMDSTR07A | 218–335 | ttctggttttctggtctg/gaatcaatctctctgtcaag |
STR-44 | 180–200 | tccaacattggaaatcacatttcaa/tcatcacaaatagatgtttcacag |
STR-45 | 152–178 | gaggctataattctttaactttggc/ctctttccctctttattcatgttac |
STR-49 | 223–260 | cgtttaccagctcaaaatctcaac/catatgatacgattcgtgttttgc |
STR-50 | 232–244 | aaggttcctccagtaacagatttgg/tatgctacatagtatgtcctcagac |
3′MP1P | 65–81 | atgatcagagtgagtaatcggttgg/atatcgatctagcagcaggaagctgaatg |
D21S1919 | 168–198 | agcggactgcatgaatcaca/gcgtctatcactctcatgttccta |
D21S263 | 194–229 | tacaagttaagggtgaaattggcta/gcgtcttcatcagcaagcgtcctctta |
D21S1914 | 258–280 | ccttctgtcacattatgaggaaaca/gcgtcttaaaggtatgattcactaaa |
D21S1255 | 308–329 | ctcttgattatgccacatagac/gcgtcttgctcgcagatgtttatta |