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Table. 3 Comparison of the results of the conventional test with those of the RT-PCR-based NIPT

From: Novel method of real-time PCR-based screening for common fetal trisomies

  Non-NIPT screening testa (n = 924) RT-PCR-based NIPT (n = 924) P
Sensitivity 5/5 100% 5/5 100% (–)
Specificity 834/918 91% 905/918 99%  < 0.0001
Screening positive rate 89/924 10% 18/924 1.9%  
  NGS based NIPT (n = 51) RT-PCR-based NIPT (n = 51) P
Sensitivity 3/3 100% 3/3 100% (–)
Specificity 48/48 100% 47/48 98% NS
Screening positive rate 3/51 6% 4/51 8%  
  Diagnostic test or no screening testb (n = 48) RT-PCR-based NIPT (n = 48) P
Sensitivity N/A 12/13 92% (–)
Specificity N/A 35/35 100% (-)
Screening positive rate N/A 12/48 25%  
  1. NIPT, non-invasive prenatal test; RT-PCR, real-time polymerase chain reaction; NGS, next generation sequencing; N/A, not applicable; NS, non-specific
  2. aIncluding 2 nuchal translucency, 14 Quad tests, 905 integrated tests, and 3 sequential tests (one had a sequential test, but the result was unknown)
  3. b47 performed an invasive diagnostic test as the primary test while 1 patient did not undergo screening test