Fig. 7
Downregulation of HELLS suppresses pancreatic cancer cells proliferation, colony formation, and affects cell cycle distribution. A The expression of HELLS in pancreatic cancer cell lines and normal human pancreatic ductal epithelial cells (HPDE). The grouping of blots cropped from different parts of the same gel, or from different gels, fields, or exposures was divided with white space. B Verification at mRNA and protein levels of shHELLS in PANC-1 cells. The grouping of blots cropped from different parts of the same gel, or from different gels, fields, or exposures was divided with white space. C, D Downregulation of HELLS significantly inhibited cell proliferation, and impaired the ability of the colony formation (P < 0.05). E Cell cycle distribution under different shHELLS analyzed by flow cytometry. F A significant increase in S fraction in shHELLS group compared with the shHELLS-con group (****P = 0.0001)