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Table 2 PCR primers for directed sequencing analysis of SLC12A3 gene

From: A novel compound heterozygous variant of the SLC12A3 gene in Gitelman syndrome with diabetes and the choices of the appropriate hypoglycemic drugs: a case report

Exon Forward primer Reverse primer
1 5′-AACTCCTTCTTGGGTCCTGG-3′ 5′-TTGGGTTGCTAGTGATTGGC-3′
2 5′-CCTACCTGCCTGACTTGTGG-3′ 5′-GAGGAGAAAAACACATTTACGG-3′
3 5′-CTGAAGTGGGTGAAGAAGGG-3′ 5′-GACTGAACCAGGGAGGAGAA-3′
4,5 5′-GGTGAATGAGTAGGCAAACTGG-3′ 5′-GGGACTTGTGGGAAAGCAAT-3′
6 5′-TGGCAGGGGTGGTGCTTGAGTT-3′ 5′-TGGAGGGGATGTGGGTATGGTG-3′
7,8 5′-GCGGTCTTGTTCACTGCTATA-3′ 5′-GCCATTCTGTGGTGTCCCTC-3′
9 5′-CCGACCCGTGATCTTGGTT-3′ 5′-TCCTTGGTGAAATAGGGAAAA-3′
10,11 5′-AATGCCTGCTCGTAGGTTATTG-3′ 5′-GATGGATGGCTTCGGGTAGAG-3′
12 5′-CCACCATTCAAGCTCTACCC-3′ 5′-GGCACTATTGCTCCCATTCT-3′
13 5′-AGTATTTCTTGCTGTCATTTGTGG-3′ 5′-CTGGCTAATTTTTGTGTTTTTGTA-3′
14 5′-GGAGCTGGTGCTGTTGCTGA-3′ 5′-CACATTGGGAGGGATAAAGG-3′
15 5′-CAGCACAACCTCGGCTCACT-3′ 5′-CAGGTCTAGGCTTGGAAACTC-3′
16,17 5′-ACCACCATTCAGGGAGCCT-3′ 5′-GTTGTGCCACCAAGCCGTA-3′
18 5′-GGTTCCCCATCTCACCCCTATCC-3′ 5′-TCTTTGCTCACTGCAGCCTTCAA-3′
19 5′-GAGAACAGAAAGGGCGTGGTA-3′ 5′-AAACTGATGGGCTCTAAGGGA-3′
20 5′-GCCCTGTCAAGGAGGAACCC-3′ 5′-AGGCACCACCGTCACAAGAA-3′
21 5′-CGGCTGCTGGCTCTGCTCTGAC-3′ 5′-GCACCGCCCATCTCCCCATTTA-3′
22 5′-GTTTGCTAATGGCAGAGCGG-3′ 5′-GAGCTAAGATGACACTGGTCCCT-3′
23 5′-CCGTTTCACTTGTCATCATCT-3′ 5′-ACATCTAGGAAGGGCTTGGAG-3′
24 5′-CACAGGTCAGTGGTTGTGGGCAA-3′ 5′-GAGTGGAAGGCAGGGTGGAGGAT-3′
25 5′-TTGGAGGAGGTGAGCTTGGTG-3′ 5′-CGTGGGTCCAGTAGGAACAGC-3′
26 5′-GTCCATTCTGGGTCAGGTTTG-3′ 5′-TGCAGCTCCATCTGCTATTTC-3′