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Fig. 1 | BMC Medical Genomics

Fig. 1

From: RNA sequencing of blood in coronary artery disease: involvement of regulatory T cell imbalance

Fig. 1

Schematic of study design. Patients presenting for elective invasive coronary angiography (ICA) due to suspicion of CAD were consented to determine whether RNA transcripts in blood could serve as biomarkers for CAD. Typically, patients reported chest pain or shortness of breath upon exertion. The results of the angiogram divided the patients into groups with little to no coronary blockage (< 20%, LOW CAD), or patients in which coronary blockage was detected (> 20%, MID+ CAD). The blood from the patients was frozen in Tempus blood RNA preservative, thawed, extracted for RNA, depleted of residual genomic DNA and ribosomal RNA, and genome-wide RNA transcript counting was performed by RNAseq. The two groups were compared to identify transcripts unique to the CAD patients. Images were created by the authors

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