Fig. 4

Clinical versus RNA predictors of CAD. a Conventional clinical predictors of CAD plotted for each group in the upper panel, showing Age (decades/10), Sex (%Male), Symptom type (typical/atypical), Diabetes (%), Hypertension (HTN, %), Family History of CAD (%), and current Smoking (%). A cumulative CAD risk score is calculated for each patient based on the method of Min et al. and divided by 10 for graphic purposes. The relationship between the cumulative risk score and CAD was calculated by the Receiver Operator Characteristic (ROC) and a confusion matrix to identify the accuracy of the method (lower left). b The performance of 7 RNA transcripts as their gene symbols (i.e. DGKA, DLG1) expressed as the RPKM by CAD group. A cumulative score was computed expressing each transcript as a ratio to the mean of its expression in the entire group, to prevent highly expressed transcripts from being over-represented. For graphic purposes, the TRIM28 and Cumulative scores are /10. In the lower panel, the relationship between the cumulative TRAC score (constant-TRAC, to create positive ROC) and angiographically-confirmed CAD is analyzed by ROC similar to the clinical model for the 48 patients in each group