Fig. 4

siRNA-mediated knockdown of CHURC1, GZF1, and RPL15 rescues ΔF508-CFTR function in CFBE cells. a Average change in transepithelial current (I_t) in response to the cAMP agonists forskolin and IBMX (F&I) and the CFTR inhibitor GlyH-101 under open circuit conditions was measured in CFBE cells. Three siRNAs per gene were pooled and CFBE cells were reverse-transfected using Lipofectamine RNAiMAX and grown on microporous Transwell membranes seven days prior to the electrophysiology measurements. Error bars indicate standard error. Statistical significance compared to the scrambled control was determined by Brown-Forsythe ANOVA and post-hoc Benjamini–Hochberg multiple testing correction (*p < 0.05). n = 4–6 per gene. CHURC1, GZF1, and RPL15 are highlighted in green. b Representative transepithelial current tracings showing CFTR-dependent chloride current in CFBE cells treated with a scrambled control or siRNA targeting GZF1. The Y-axis represents transepithelial current in µA and the X-axis represents time in seconds