Table 2 PSORT predictions of HCAR3 wildtype and mutant, p.Gln373Lysfs*82
HCAR3 protein | ER Membrane Retention Signals: | NUCDISC: discrimination of nuclear localization signals | Tripeptide SKL-motif | Prenylation Motif | k-NN (k-nearest neighbors algorithm) | |||
|---|---|---|---|---|---|---|---|---|
Wildtype | KKXX-like motif in the C-terminus: NONE | content of basic residues: 9.8% | peroxisomal targeting signal in the C-terminus: NONE | CCIE | 55.6%: endoplasmic reticulum | 22.2% vacuolar | 11.1% nuclear | 11.1% Golgi |
Mutant | KKXX-like motif in the C-terminus: KMGK | content of basic residues: 11.5% | peroxisomal targeting signal in the C-terminus: GKL | NONE | 77.8%: endoplasmic reticulum | 11.1% vacuolar | 0.0% nuclear | 11.1% Golgi |