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Table 1 The result of NGS and Sanger sequencing for TERT promoter region

From: Direct comparison of the next-generation sequencing and iTERT PCR methods for the diagnosis of TERT hotspot mutations in advanced solid cancers

No Tumor DNA concentration (ng/μl) NGS data Sanger sequencing
TMB MSI NGS TERT VAF (%) TERT TD TERT TV (%)
1 Liver 20 TMB-low MSS C228T 45.6 456 80 C228T
2 Liver 14 TMB-low MSS C228T 25.9 201 80 C228T
3 Liver 9 TMB-low MSS C228T 25.6 78 70 C228T
4 Liver 31 TMB-high MSS C228T 15.4 259 20 C228T
5 Liver 22 TMB-low MSS C228T 36.0 114 90 C228T
6 Liver 27 TMB-low MSS C228T 41.7 211 80 C228T
7 Liver 9 TMB-low MSS C228T 7.7 78 60 C228T
8 Liver 33 TMB-high MSS C228T 44.7 94 40 C228T
9 Liver 6.4 TMB-low MSS C228T 26.8 112 60 C228T
10 Liver 16 TMB-low MSS C228T 34.1 552 60 C228T
11 Liver 11 TMB-low MSS C228T 24.7 178 70 C228T
12 Liver 47 TMB-low MSS C228T 28.8 66 20 C228T
13 Liver 26 TMB-low MSS C250T 27.9 1223 70 C250T
14 Liver 22 TMB-low MSS C228T 38.1 578 60 C228T
15 Liver 47 TMB-low MSS C250T 12.0 875 60 C250T
16 Liver 35 TMB-low MSS C228T 56.2 441 80 C228T
17 Liver 153 TMB-low MSS C228T 28.6 398 70 C228T
18 Liver 83 TMB-low MSS C228T 24.4 586 80 C228T
19 Liver 135 TMB-high MSS C250T 38.2 728 80 C250T
20 Liver 121 TMB-low MSS C228T 34.7 254 60 C228T
21 Liver 140 TMB-low MSS C228T 20.9 134 70 C228T
22 Liver 139 TMB-low MSS C228T 49.4 237 60 C228T
23 Melanoma 50 TMB-high MSS C250T 11.4 500 60 C250T
24 Melanoma 98 TMB-low MSS C250T 28.8 351 80 C250T
25 Melanoma 52 TMB-high MSS C228T 23.8 395 40 C228T
26 Melanoma 76 TMB-low MSS C228T 54.6 227 70 C228T
27 Melanoma 76 TMB-low MSS C228T 26.9 93 70 C228T
28 Melanoma 190 TMB-low MSS C250T 41.5 585 40 C250T
29 Melanoma 138 TMB-high MSS C250T 57.5 315 30 C250T
30 Melanoma 49 TMB-low MSS C228T 53.4 251 80 C228T
31 Melanoma 195 TMB-high MSS C250T 21.7 359 80 C250T
32 Melanoma 137 TMB-low MSS C228T 20.8 226 50 C228T
33 Melanoma 138 TMB-low MSS C250T 29.3 399 40 C250T
34 Melanoma 136 TMB-low MSS C250T 47.5 385 40 C250T
35 Pancreatobiliary 188 TMB-low MSS C228T 34.6 81 60 C228T
36 Pancreatobiliary 456 TMB-high MSS C228T 20.5 234 50 C228T
37 Pancreatobiliary 50 TMB-low MSS C228T 12.1 239 50 C228T
38 Pancreatobiliary 61 TMB-low MSS C228T 10.8 249 25 C228T
39 Pancreatobiliary 159 TMB-low MSS C228T 11.7 137 10 C228T
40 Pancreatobiliary 15 TMB-low MSS C228T 33.1 130 90 C228T
41 Pancreatobiliary 17 TMB-high MSS C228T 31.7 145 70 C228T
42 Pancreatobiliary 19 TMB-low MSS C250T 16.9 705 40 C250T
43 Pancreatobiliary 57 TMB-low MSS C228T 36.6 544 50 C228T
44 Pancreatobiliary 11 TMB-low MSS C228T 45.9 270 60 C228T
45 Pancreatobiliary 22 TMB-high MSS C250T 18.0 666 60 C250T
46 Pancreatobiliary 45 TMB-low MSS C228T 21.7 337 30 C228T
47 Pancreatobiliary 41 TMB-low MSS C228T 29.3 246 30 C228T
48 Pancreatobiliary 18 TMB-low MSS C250T 12.8 639 60 C250T
49 Pancreatobiliary 23 TMB-high MSS C228T 25.1 470 40 C228T
50 Pancreatobiliary 36 TMB-high MSS C228T 24.9 503 70 C228T
51 Pancreatobiliary 36 TMB-low MSS C228T 19.1 236 40 C228T
52 Pancreatobiliary 11 TMB-low MSS C228T 33.1 366 30 C228T
53 Pancreatobiliary 47 TMB-high MSS C228T 39.2 199 80 C228T
54 Urinary 20 TMB-low MSS C228T 32.6 331 70 C228T
55 Urinary 81 TMB-low MSS C228T 22.7 238 70 C228T
56 Urinary 74 TMB-low MSS C250T 16.5 388 60 C250T
57 Urinary 172 TMB-high MSS C250T 19.7 117 70 C250T
58 Urinary 28 TMB-high MSS C228T 32.8 125 80 C228T
59 Urinary 30 TMB-high MSS C228T 19.5 41 30 C228T
60 Urinary 32 TMB-high MSS C228T 30.9 628 80 C228T
61 Urinary 70 TMB-low MSS C228T 21.7 60 60 C228T
62 Urinary 24 TMB-high MSS C250T 8.7 69 10 C250T
63 Urinary 35 TMB-low MSS C250T 22.3 197 70 C250T
64 Urinary 58 TMB-high MSS C228T 37.9 103 60 C228T
65 Urinary 54 TMB-low MSS C228T 14.2 106 60 C228T
66 Urinary 31 TMB-high MSS C250T 24.7 515 70 C250T
67 Urinary 47 TMB-high MSS C228T 28.1 740 70 C228T
68 Urinary 38 TMB-high MSS C228T 46.9 260 40 C228T
69 Urinary 43 TMB-low MSS C250T 49.2 1140 90 C250T
70 Urinary 25 TMB-low MSS C250T 47.6 993 70 C250T
71 Urinary 49 TMB-low MSS C228T 34.3 134 40 C228T
72 Urinary 22.4 TMB-low MSS C228T 53.8 409 35 C228T
73 Urinary 32 TMB-low MSS C228T 10.8 510 50 C228T
74 Urinary 27 TMB-low MSS C228T 25.3 301 20 C228T
75 Urinary 63 TMB-low MSS C228T 18.4 87 90 C228T
76 Urinary 42 TMB-low MSS C228T 47.6 410 70 C228T
77 Urinary 38 TMB-high MSS C228T 24.6 272 20 C228T
78 Urinary 63 TMB-low MSS C228T 21.1 95 70 C228T
79 Urinary 51 TMB-high MSS C228T 37.1 329 40 C228T
80 Urinary 169 TMB-low MSS C228T 30.1 332 80 C228T
81 Urinary 30 TMB-low MSS C228T 27.9 219 70 C228T
82 Urinary 15 TMB-low MSS C228T 18.1 205 40 C228T
83 Urinary 27 TMB-low MSS C228T 32.1 545 40 C228T
84 Urinary 39 TMB-low MSS C228T 18.1 276 70 C228T
85 Brain 51 TMB-low MSS C228T 51.6 31 70 C228T
86 Colon 64 TMB-high MSS C228T 28.3 152 40 C228T
87 Colon 76 TMB-low MSS C228T 29.4 286 50 C228T
88 Colon 87 TMB-low MSS C228T 31.8 63 90 C228T
89 Colon 61 TMB-high MSS C228T 34.3 429 60 C228T
90 Colon 18 TMB-low MSS C228A 38.8 268 40 C228A
91 Colon 30 TMB-low MSS C228T 38.9 779 30 C228T
92 Colon 42 TMB-low MSS C228A 53.2 139 60 C228A
93 Head and neck 54 TMB-low MSS C228T 25.3 95 20 C228T
94 Head and neck 66 TMB-low MSS C228T 31.6 493 70 C228T
95 Head and neck 77 TMB-low MSS C250T 85.1 215 50 C250T
96 Lung 90 TMB-low MSS C228T 20.2 134 60 C228T
97 Lung 37 TMB-high MSS C228T 26.2 84 40 C228T
98 Sarcoma 26 TMB-low MSS C228T 50.0 118 90 C228T
99 Sarcoma 14 TMB-low MSS C228T 56.6 53 80 C228T
100 Sarcoma 37 TMB-low MSS C250T 67.5 437 50 C250T
101 Sarcoma 45 TMB-low MSS C228T 70.9 117 25 C228T
102 Skin 136 TMB-low MSS C228T 37.8 394 20 C228T
103 Thyroid 163 TMB-low MSS C228T 45.6 204 80 C228T
  1. NGS next-generation sequencing, TERT telomerase reverse transcriptase, VAF variant allele frequency, TD total read depth, TV tumor volume