Table 3 Comparison of iTERT PCR with NGS
| Â | NGS | iTERT PCR |
|---|---|---|
Quality of DNA | Limited by damaged DNA in certain cases Needed high-quality DNA | Rarely limited by damaged DNA |
Quantity of DNA | Needed the amount of DNA required for downstream NGS preparation steps (50 ~ 120 ng) | Relatively 'small' amount of DNA is required (< 50 ng) |
Test time | Requires more time for the preparation of library preparation (2 days) | Time-saving and easy PCR preparation (< 3 h) |
Costs (per case) | £570 | £30 |
Interpretation | Very complex, and its interpretation requires expert bioinformatics assistance | Easy to analyze PCR-Sanger sequencing results |