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Table 3 Comparison of iTERT PCR with NGS

From: Direct comparison of the next-generation sequencing and iTERT PCR methods for the diagnosis of TERT hotspot mutations in advanced solid cancers

  NGS iTERT PCR
Quality of DNA Limited by damaged DNA in certain cases Needed high-quality DNA Rarely limited by damaged DNA
Quantity of DNA Needed the amount of DNA required for downstream NGS preparation steps (50 ~ 120 ng) Relatively 'small' amount of DNA is required (< 50 ng)
Test time Requires more time for the preparation of library preparation (2 days) Time-saving and easy PCR preparation (< 3 h)
Costs (per case) £570 £30
Interpretation Very complex, and its interpretation requires expert bioinformatics assistance Easy to analyze PCR-Sanger sequencing results