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Table 3 Comparison of iTERT PCR with NGS

From: Direct comparison of the next-generation sequencing and iTERT PCR methods for the diagnosis of TERT hotspot mutations in advanced solid cancers

Quality of DNA Limited by damaged DNA in certain cases Needed high-quality DNA Rarely limited by damaged DNA
Quantity of DNA Needed the amount of DNA required for downstream NGS preparation steps (50 ~ 120 ng) Relatively 'small' amount of DNA is required (< 50 ng)
Test time Requires more time for the preparation of library preparation (2 days) Time-saving and easy PCR preparation (< 3 h)
Costs (per case) £570 £30
Interpretation Very complex, and its interpretation requires expert bioinformatics assistance Easy to analyze PCR-Sanger sequencing results