Table 2 Primer sequence list for the three circRNAs for qPCR
Gene | Primer sequence | Annealing temp (°C) | Product size (bp) |
|---|---|---|---|
GAPDH(HUMAN) | F:5′GGGAAACTGTGGCGTGAT3′ R:5′GAGTGGGTGTCGCTGTTGA3′ | 60 | 299 |
hsa_circRNA_009012 | F:5′CCTAGAAGCCACCCAAACTG3′ R:5′CAGGACTTCAGGAAAGCACC3′ | 60 | 60 |
hsa_circRNA_101328 | F:5′GGACGGCGTCACCAACCTA3′ R:5′GCACGCATTCTTTCTGGACAT3′ | 60 | 150 |
hsa_circRNA_058230 | F:5′TGGATGGGGAGCCCTACAAG3′ R:5′CCAGGTGCGGGTGTACAGG3′ | 60 | 94 |