Fig. 1
From: Inter-assay variability of next-generation sequencing-based gene panels
Study design. A total of 30 samples were classified into three groups according to sample type and DNA library concentration: Group FF (n = 10, yellow), fresh frozen (FF) tissue samples; Group FFPE-H (n = 10, green), FFPE samples with DNA library concentrations ≥ 5 nM; and Group FFPE-L (n = 10, purple), FFPE samples with DNA library concentrations < 5 nM. Identical DNA samples were analyzed using tumor-only (TO) and tumor–normal (TN) panel assays. Additional FFPE samples were sliced from the same FFPE block (Group FFPE-H and FFPE-L, n = 20) and submitted for TN panel assay