Fig. 1

Overview of the study. 269 cells from four tumor tissues and two thrombi tissues were extracted. HBV genome sequence enrichment was performed after whole genome amplification on the single cell DNA genome. Pair-end sequencing was used. A pipeline was developed for HBV integration identification and CNV inference. Tumor clones were inferred based CNV profile. Association between HBV integration and CNV was assessed based on clone inference and phylogenetic tree. Key CNVs differentiate two clones were identified with phylogenetic tree. Statistical test was performed on the key genetic regions while considering only cells belonging to related clones. Images in the figure are drew by the authors