Table 1 The sequence of specific primers for studying six candidate promoter regions of VitD R, Claudin 2, CaSR for high‐resolution melting analysis
Gene | Locus | Primers (Forward and Reverse) | Length of Amplicon | Tann (degrees Celsius) | Number of covered CpG dinucleotides within the amplicon |
|---|---|---|---|---|---|
CLDN14 | a | F: AGTTTATAGAGGTAATTTTATTTTG R: CTACACACCAACTCATAACC | 272 bp | 50 | 18 CpG sites |
CLDN14 | b | F: GGTTATGAGTTGGTGTGTAGT R: TATTTAAATCACACTTAAAAT | 214 bp | 52 | 5 CpG Sites |
CaSR | a | F: GTGTTAGGGGTTAGGGATAAGGATA R: TCATTCTACAAAACTCAAATCAAAC | 218 bp | 59 | 11 CpG Sites |
CaSR | b | F: AGAATGAGTAAGAGTTTGGGTA R: CTCTTCCCTAACCCCTACTCCT | 175 bp | 55 | 8 CpG Sites |
VitD R | a | F: TAATAGTATTAGTGGGAGTGGGGAT R: AAATCCTAAAATAAACAAACACACC | 215 | 56 | 5 CpG Sites |
VitD R | b | F: AGTTTGGGGATAGGGGTGAGGTTA R: CACTTATTCACCTCCACACACCTAC | 168 | 55 | 11 CpG Sites |