Analysis of TMIE gene mutations including the first large deletion of exon 1 with autosomal recessive non-syndromic deafness

Table 1 Primer sequences and products size of the TMIE gene

Family	Exon number	Primer name	Primer sequence (5′–3′)	Product size ( bp)
1	Upstream Exon 1	F1	5′- AGAATCGAATTGGAAGGCAC-3′	184
	Upstream Exon 1	R1	5′-TTAAGGCGAACATCCTGAAAG-3′	184
	Upstream Exon 1	F2	5′-CTGTGAGCGGGGTATCTTAC-3′	326
	Upstream Exon 1	R2	5′-CGCAACTCCAGAGCAAC-3′	326
	Downstream Exon 1	F3	5′-CAGCCCACAGATCCTCTG-3′	225
	Downstream Exon 1	R3	5′-TAAATAAGGACAGACACGAGAAGTC-3′	225
	Multiplex Exon 1	F1	5′-AGAATCGAATTGGAAGGCAC-3′	184
		R1	5′-TTAAGGCGAACATCCTGAAAG-3′
		F2	5′-CTGTGAGCGGGGTATCTTAC-3′
		R3	5′-TAAATAAGGACAGACACGAGAAGTC-3′	1111
2	Exon 2	F4	5́-CTCCCACTTCAAGTACCTGGCTC-3́	616
	Exon 2	R4	5́-CACGTCCTCGTCCCAGTCC-3́
3	Exon 3	F5	5́-CCATTCCTTGGGTCTCTGAA-3́	288
3	Exon 3	R5	5́-AGCAGAGGAACAGGGTGAC-3́

ISSN: 1755-8794