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Table 1 Primer sequences and products size of the TMIE gene

From: Analysis of TMIE gene mutations including the first large deletion of exon 1 with autosomal recessive non-syndromic deafness

Family Exon number Primer name Primer sequence (5′–3′) Product size ( bp)
1 Upstream
Exon 1
F1 5′- AGAATCGAATTGGAAGGCAC-3′ 184
R1 5′-TTAAGGCGAACATCCTGAAAG-3′
Upstream
Exon 1
F2 5′-CTGTGAGCGGGGTATCTTAC-3′ 326
R2 5′-CGCAACTCCAGAGCAAC-3′
Downstream
Exon 1
F3 5′-CAGCCCACAGATCCTCTG-3′ 225
R3 5′-TAAATAAGGACAGACACGAGAAGTC-3′
Multiplex
Exon 1
F1 5′-AGAATCGAATTGGAAGGCAC-3′ 184
R1 5′-TTAAGGCGAACATCCTGAAAG-3′  
F2 5′-CTGTGAGCGGGGTATCTTAC-3′  
R3 5′-TAAATAAGGACAGACACGAGAAGTC-3′ 1111
2 Exon 2 F4 5́-CTCCCACTTCAAGTACCTGGCTC-3́ 616
  R4 5́-CACGTCCTCGTCCCAGTCC-3́  
3 Exon 3 F5 5́-CCATTCCTTGGGTCTCTGAA-3́ 288
R5 5́-AGCAGAGGAACAGGGTGAC-3́