Fig. 1
From: TMBur: a distributable tumor mutation burden approach for whole genome sequencing
Schematic diagram of the Nextflow workflow for the singularity container used in the TMBur pipeline. TMBur is a portable software package that contains multiple individual components, including variant caller tools (Manta-Strelka2, Mutect2, and MSIsensor2) and resources (the human genome reference sequence [hg19] and reference annotations [SNPEff Ens75]), all used to provide consistent tumor mutation burden (TMB) counts. The workflow allows for the analysis of raw, whole-genome data from pairs of samples (tumor and normal) and can be conducted with multiple pairs simultaneously