Fig. 3

Correlation of pre-capture library concentration with bioinformatics metrics for all samples excluding FFzn controls. Scatter plot of RNA library concentration (ng/ul) with three bioinformatics metrics. Samples colored in red/green indicates qc failed/pass samples according to sample-wise median correlation, number of detected genes with TPM higher than 4, number of gene mapped reads. Spearman correlation and p value are shown on the upper right of each panel. A smooth line was fit to the data using loess and 95% confidence interval was also indicated as grey shaded area. a Scatter plot of library concentration with sample wise median correlation. Dashed line indicates a correlation of 0.75. b Scatter plot of library concentration with number of genes with transcript per million (TPM) higher than 4. Dashed line indicates 11,400 genes. c Scatter plot of library concentration with total number of gene mapped reads. Dashed line indicates 25 million gene mapped reads. d Scatter plot of library concentration with estimated local/regional failure rate calculated based on bioinformatics metrics within each window of library concentration