Fig. 2
From: Ultra-sensitive molecular detection of gene fusions from RNA using ASPYRE
The ASPYRE-Lung RNA assay approaches the single molecule limit. Synthetic RNA oligonucleotides were quantified by dPCR, and added to reactions at the specified number of copies, with 12 replicates. Detection of one, two, three, six or nine copies of target in the multiplexed ASPYRE-Lung assay, for the following targets: EML4ex13-ALKex20, ETV6ex5-NTRK3, CD74ex6-ROS1ex32, and TRIM33ex14-RETex12. Two negative controls were also analyzed: water, and total human lung RNA.