Fig. 2

Survival of NALM-6 cells and expression of the ACOT7–NPHP4. a The transfection of the plvx-ACOT7-NPHP4-Flag (plvx-A-N-Flag) or vector plasmids into NALM-6 cells showed that NALM-6 cells carrying ACOT7–NPHP4 (red line) presented a significant chemoresistance to doxorubicin after a 24-h exposure compared with NALM-6 cells carrying the vector plasmid (black line), as assessed using the cell counting kit-8 assay (mean ± SEM is displayed). **P < 0.01; ***P < 0.001; ****P < 0.0001. The expression of plvx-A-N-Flag in NALM-6 cells was tested via western blotting using an anti-Flag antibody. b The primary lymphoblastic leukemia cells from the patient were decreased after induction chemotherapy, as analyzed by flow cytometry in the bar chart on the left. The transcripts of ACOT7–NPHP4 were decreased after induction therapy, as assessed by quantitative real-time PCR in the bar chart on the right