Table 1 Methylation-sensitive high-resolution melting primers, designed for promoter regions, and their amplicon information
Gene name | Sequence (5′ → 3′) | Ta˚C | Accession number | Number of CpG-sites/amplicon length |
|---|---|---|---|---|
DIO3 | F: GAG GGT ATT GTA GTA AGG TGT ATT R: AAA ACC CAA CCC ACC AAA TTC | 58 | NC_000014.9 | 18/271 bp |
THRB | F: GTG TTA TTA GTT TGA TTA TTT GTT R: CTA TTC CAC CAC TAT CCA C | 54 | NC_000003.12 | 11/128 bp |