Fig. 6
Transcriptional response of mRNAs in the core module in vitro. A Highlighted DE microRNAs (log2 |FC| ≥ 0.5, padj ≤ 0.05) having a consistent direction of the change in expression in PBMCs in THP-1/miR-20a-5p and THP-1/miR-340-5p groups. B Fold changes in the levels of DE microRNAs having a consistent direction of the change in expression in PBMCs in THP-1/miR-20a-5p and THP-1/miR-340-5p groups. C Fold changes of ten macrophage polarization marker genes before and after the mimics transfection (insignificant changes were marked as “na”). D Highlighted DE microRNAs (log2 |FC| ≥ 0.5, padj ≤ 0.05) having a consistent direction of the change in expression in PBMCs in LX-2/miR-20a-5p and LX-2/miR-340-5p groups. E Fold changes in the levels of DE microRNAs having a consistent direction of the change in expression in PBMCs between the LX-2/miR-20a-5p and LX-2/miR-340-5p groups. F Fold changes of ten HSC activation marker genes before and after the mimics transfection (insignificant changes were marked as “na”). G Dot plot showing the results of functional analysis of the expression changes of the core module in THP-1 after the mimic transfection of miR-20a-5p and miR-340-5p. H Dot plot showing the results of functional analysis of the expression changes of the core module in LX-2 after the mimic transfection of miR-20a-5p and miR-340-5p. Numbers of replicates for RNA-Seq in each group (n = 3 in the THP-1/miR-20a-5p, THP-1/miR-340-5p, LX-2/miR-20a-5p, LX-2/miR-340-5p and NC groups, respectively)