Fig. 5From: Does the LHPP gene share a common biological function in pancancer progression?The biological functions of the LHPP gene were determined in gastric cancer. A LHPP protein expression level was detected via IHC in cancer tissues vs. normal tissues. B LHPP protein expression was downregulated in cancer tissues compared to their corresponding normal tissues. C and D The effect of LHPP upregulation on the proliferation of HGC-27 cells was determined via a colony formation assay. A significant difference was observed by using GraphPad Prism 6 software. (E) A Transwell assay was utilized to examine the migration and invasion (matrix gel 50–80 μl, dilution 1:8, 8-μm pore) abilities of HGC-27 cells after overexpressing LHPP protein. F The cell cycle-related and EMT-related biomarkers, such as CDK4, CyclinD1, NME1, BCL-2, Vimentin, Snail, Slug and Twist1, were evaluated by using a Western blot assay. G The relative expression of LHPP protein was examined between gastric cancer tissues and matched normal tissues via ImageJ and GraphPad Prism 6 software. H The relative expression levels of related proteins were calculated using ImageJ and GraphPad Prism 6 software. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001Back to article page