Genome-wide methylation profiling identify hypermethylated HOXL subclass genes as potential markers for esophageal squamous cell carcinoma detection

Table 2 Primers used for methylation-specific PCR

Gene	Primer	Sequence (5′ –> 3′)	Position	Length (bp)	CpG sites
HOXD1	Forward	CCCCGTTGTAGGTAAATTCGTC	Chr2:177054528–177054672	144	10
HOXD1	Reverse	GGGACTATCTCGATACGCCGA	Chr2:177054528–177054672	144	10
HOXC10	Forward	TATTTGACGCGAGAGCGTCG	Chr12: 54383074–54383217	147	7
HOXC10	Reverse	TTAAAATTAAAAATCAATTCCCG	Chr12: 54383074–54383217	147	7

ISSN: 1755-8794