Table 1 Primer sequences used to analyze the exons and the cDNA of FAH gene
Primer name | Primer sequence (F) | Primer sequence (R) | Product size (bp) |
|---|---|---|---|
FAH-exon 1 | CGGTGAGACCAAAAGTCAGGT | GCAAACCTGCGGACAATGAG | 478 |
FAH-exon 2 | ACCGCACAACTGAACTACCC | GTTGGCATCCACAGTAAGTGC | 590 |
FAH-exon 3 | TGGAGTGTGCCTCTACTGGA | CCGTGACTCAGAATGGCACT | 533 |
FAH-exon 4 | ATTCTGAGTCACGGCTTGGC | ATTCTGAGTCACGGCTTGGC | 590 |
FAH-exon 5 | CACAGGGACAAGGGAGAAGTC | GAACTTGGCAGCTCCTGAGAC | 557 |
FAH-exon 6&7 | AGCTCTGATGCCCTGCATT | CTGCCGATGTGGCTGAAGAG | 423 |
FAH-exon 8 | CACCACTGCACTCTGCTTTC | TCACCTGCCACTTTTGACCT | 400 |
FAH-exon 9 | GGACCTCTGTCCTTGGCATT | GAGCTTCCCTCCTGATGGTCT | 420 |
FAH-exon 10 | TCCTGCTGTCTCAGACCCTC | AGACGAGCCACACACATCTC | 336 |
FAH-exon 11 | GTGGGAGGAGGAAGTGATGA | AAAAGAGACAGGCCATGGAA | 402 |
FAH-exon 12 | AAGGGACTGGAGAGAGCTGG | GTGAGATACACCCACCTCGG | 372 |
FAH-exon 13 | TGGTGCATGTGTCACTCACT | AGCTAGAACAGTGCATGGCG | 583 |
FAH-exon 14 | GCTTCCGTGGAGGGTTATTCT | AGGAGGCCTGGGATGTCTAAT | 539 |
FAH-cDNA-1 | GTCCTTCATCCCGGTGGC | GGTAGCCCACTGGTAAGTGC | 476 |
FAH-cDNA-2 | CACCTTCCAGCCACCATAGG | TCGTCATGGCACAGATACGG | 527 |
FAH-cDNA-3 | TGGGAGTATGTCCCTCTCGG | TCATGATGGCAGGAGAGCAG | 537 |