Table 1 Primer sequences used for qRT-PCR amplification
Gene | Forward primer (5’→3’) | Reverse primer (5’→3’) |
|---|---|---|
COPS7A | CCACACTCATCCATCAGGTGCT | CGGAAGGTAGAGGCAAAGTCAC |
CUL3 | TCGACAGCTCACACTCCAGCAT | GTGCTTCCGTGTATTAGAGCCAG |
KLHL7 | TTGTGTGACGTGATCCTCATGG | TATCAGGTTCAGCATCTTTGAGTTC |
NEDD4 | CAGAAGAGGCAGCTTACAAGCC | CTTCCCAACCTGGTGGTAATCC |
GAPDH | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA |
qRT-PCR, quantitative real‐time polymerase chain reaction. | ||