Fig. 4

Relative expression levels of methylation, miRNA, and mRNA. (A-G) Orange column is qPCR data and blue sequencing data. a Sequencing and qPCR levels of has-miR-194-3p. b Sequencing and qPCR levels of has-miR-200a-3p. c Sequencing and qPCR levels of has-let-7b-5p. Because the expression level has too large gap, the left y coordinate axes is for sequencing data and the right for qPCR result. d Sequencing and qPCR levels of RAS. e Sequencing and qPCR levels of MAPK1. f Sequencing and qPCR levels of FOS. Because the expression level has too large gap, the left y coordinate axes is for sequencing data and the right for qPCR result. B Sequencing and qPCR levels of FAS. h Methylation levels of miR-194, miR-200a, let-7b with pyrosequencing. *P < 0.05, **P < 0.01, significantly different from the control group in sequencing. ^#P < 0.05, ^##P < 0.01, significantly different from the control group in qPCR