Table 1 Primer sequences used for FSHR DNA Sanger’s sequencing
position | forward primer | reverse primer | amplicon lenght | annealing temperature |
|---|---|---|---|---|
Exon 1 | 5’-CATCCCTTGGTGGGTCACATG-3’ | 5’-AAATGCCAGCCATGCAGTTG-3’ | 336 bp | 59 °C |
Exon 2 | 5’-AGACAGGATGAAAAGAGAGAATG-3’ | 5’-TTGAGGCATTCACTCACAGC-3’ | 263 bp | 57 °C |
Exon 3 | 5’-GCCACAGCCTTCGACTTATTC-3’ | 5’-GCCTCCCAGGAATGTAGAAG-3’ | 367 bp | 59 °C |
Exon 4 | 5’-GCACAGCTTAGTGTGATAAAAGGC-3’ | 5’-GTGGGGGTACCAAACTACATG-3’ | 302 bp | 59 °C |
Exon 5 | 5’-CTCTGAGGAATCAACAGCTTTTAAG-3’ | 5’-GGGCAAGACAGATACTGAG-3’ | 334 bp | 53 °C |
Exon 6 | 5’-GTCTGCAATTCCATTTGTAAGAAC-3’ | 5’-ATCAAATGTTACTCTGTTGG-3’ | 327 bp | 50 °C |
Exon 7–8 | 5’-TACAGCAATAAATCAGTCTTCCTCC-3’ | 5’-GAGAGTTGACTTCTAACTTACAC-3’ | 489 bp | 59 °C |
Exon 9 | 5’-GAAGGACCAGGACTCCTACAGAAC-3’ | 5’-TGCCTGAGCAGGGCTTAAAG-3’ | 415 bp | 57 °C |
Exon 10, 5’ segment | 5’-GCTATACTGGATCTGAGATG-3’ | 5’-ATCCAGCCCATCACCATGAC-3’ | 680 bp | 62 °C |
Exon 10, 3’ segment | 5’-AGCTGGACTGCAAGGTGCAG-3’ | 5’- TGTAGAAGCACTGTCAGCTC-3’ | 752 bp | 59 °C |